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1. Dougherty, J., Gomer, C. J., Henderson, B. W., Jori, G., Kessel, D., Korbelik, M., Moan, J., and Peng, Q. Review. Photodynamic therapy. J. Natl. Cancer Inst., 90: 889 905, Dougherty, T. J., Cooper, M. T., and Mang, T. S. Cutaneous phototoxic occurrences in patients receiving Photofrin. Lasers Surg. Med., 10: 485 488, Overholt, B. F., and Panjehpour, M. Photodynamic therapy in Barrett's esophagus. J. Clin. Laser Med. Surg., 14: 245249, 1996. Nseyo, U. O., Shumaker, B., Klein, E. A., and Sutherland, K. Photodynamic therapy using porfimer sodium as an alternative to cystectomy in patients with refractory transitional cell carcinoma in situ of the bladder. Bladder Photofrin Study Group. J. Urol., 160: 39 44, Peng, Q., Warloe, T., Berg, K., Moan, J., Kongshaug, M., Giercksky, K. E., and Nesland, J. M. 5-aminolevulinic acid-based photodynamic therapy: clinical research and future challenges. Cancer Phila. ; , 79: 22822308, 1997. Peng, Q., Berg, K., Moan, J., Kongshaug, M., and Nesland, J. M. 5-aminolevulinic acid-based photodynamic therapy: principles and experimental research. Photochem. Photobiol., 65: 235251, 1997. Peng, Q., Moan, J., Warloe, T., Nesland, J. M., and Rimington, C. Distribution and photosensitizing efficiency of porphyrins induced by application of exogenous 5aminolevulinic acid in mice bearing mammary carcinoma. Int. J. Cancer, 52: 433 443, Peng, Q., Moan, J., Farrants, G., Danielsen, H. E., and Rimington, C. Localization of potent photosensitizers in human tumor LOX by means of laser scanning microscopy. Cancer Lett., 53: 129 139, Peng, Q., Nesland, J. M., Moan, J., Evensen, J. F., Kongshaug, M., and Rimington, C. Localization of fluorescent Photofrin II and aluminum phthalocyanine tetrasulfonate in transplanted human malignant tumor LOX and normal tissues of nude mice using highly light-sensitive video intensification microscopy. Int. J. Cancer, 45: 972979, 1990. Peng, Q., Moan, J., and Nesland, J. M. Correlation of subcellular and intratumoral photosensitizer localization with ultrastructural features after photodynamic therapy. Ultrastruct. Pathol., 20: 109 129, Moan, J., Peng, Q., Rrensen, R., Iani, V., and Nesland, J. M. The biological foundations of photodynamic therapy. Endoscopy, 30: 387391, 1998.
The absence of the wild-type p53 protein. The control cell line, MCF-7, containing a wild-type of p53, undergoes apoptosis in response to numerous toxic agents, but it could be mentioned that in PDT-treated MCF-7 cells nuclear-cytoplasmic localization of p53 protein was observed [31]. These results corroborate data of Zhang et al. [32] demonstrating that introduction of wild-type p53 transfected to the p53 null HT29 human colorectal carcinoma cells made them more sensitive to induction of apoptosis after PDT. The MTT assay results revealed that the wild-type p53expressing transfected cells were approximately twofold more sensitive to PDT than non-transfected controls [32]. In our study, a restricted direct cytotoxicity of photofrin II-PDT for OvBH-1 cells was observed. We pointed out that the activity of mitochondrial enzymes was measured, but the data may not reflect cell survival .The total absence of apoptosis markers such as Bax protein and the human neoepitope cytokeratin 18 M30 ; expression in OvBH-1 cells, which are present on cells ongoing apoptosis, suggests that PDT kill cells by necrosis. Song et al. [26] revealed that hemoporfin-based PDT induced cell death of SKOV3 ovarian carcinoma cell line by necrosis. The authors [26] suggest that type of cell death induced by photocytotoxic effect of hemoporfin-PDT is cell type related. In the present study, the neoepitope cytokeratin 18 M30 ; expression accompanied the apoptosis of MCF-7 cells after photofrin II-PDT which is in agreement with the observations of Bantel et al. [3] that the diminution in neoepitope cytokeratin 18 M30 ; expression might be the marker of apoptosis. It is worth underlining that, based on the alkaline comet assay, we revealed photofrin II-PDT induced necrosis in OvBH-1 cells and apoptosis in MCF-7 cells. Several data have been published [1, 24] showing that cell death after PDT can occur by necrosis or apoptosis depending on intracellular localization of photosensitizer [24]. A few clinical studies [17, 27] have evaluated the response to platinum-based chemotherapy in patients with clear cells ovarian carcinoma. Patients with clear cell ovarian carcinoma showed a very low response rate, whereas patients with serous ovarian carcinoma had a high response to platinum therapy. We would like to point out that the patient from whom OvBH-1 cell line was derived obtained 4 cycles of cisplatin-based therapy and died 6 months after surgery. Most experimental [5, 8, 26] and clinical [12] studies were performed on serous ovarian carcinoma and showed effective results of PDT not only in vitro.
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1st dam APPROVED ANTHEM, by With Approval. Unraced. This is her second foal. Her first foal is a 2-year-old of 2006, which has not started. 2nd dam ANTHEM, by Deputy Minister. 4 wins at 3 and 4, , 027. Dam of 7 foals to race, 6 winners, including-Southern Sensation. 3 wins at 4 and 5, 2005, , 317. Buzzword. 4 wins at 3 and 4, , 520. Song of Stars. Winner at 3 and 4, , 797. Show Tune. Winner at 2 and 3, , 830. 3rd dam SWORD OF SONG, by Blade. 8 wins, 3 to 5, , 411, Red Cross S. Dam of 11 foals to race, 10 winners, including-Admiral's Intent. 2 wins, , 094, 3rd Elkwood S. [L] MTH, , 500 ; . Sword of Lucky. 6 wins, 2 to 6, 5, 015. Thurso Bay. 4 wins at 3 and 4, , 118. 4th dam ADMIRAL'S DANCER, by War Admiral. 3 wins at 2. Half-sister to LITTLE TUMBLER 6 wins, 4, 940, Astoria S., etc., dam of NATIVE TUMBLER; granddam of ICEYCINDY, 6, 554; etc. ; , SLEEPING TIGER Ohio Endurance, sire ; , Brian Boru , 067, 2nd Hyde Park S. ; , Sleepers Jinx. Dam of 8 foals to race, all winners, including-ACTUALITY. 16 wins, 2 to 8, 2, 445, Hibiscus S.-G3, Florida Junior S.-ntr, Nursery S., 2nd Golden Beach S., 3rd Princeton S.-G3, Swift S.-G3. SWORD OF SONG. Black type winner, see above. Bit of Style. Unraced. Dam of 4 foals, all winners, including-FINERY. 4 wins at 2 and 3, , 365, Vizcaya S., 3rd Begonia H., Azalea S. Producer. Granddam of MEDIEVAL ORNAMENT 5 wins, , 340, Forget Me Not S. [OR], etc. ; , Dr. Hop 5 wins, , 055 ; . Navy Nymph. Unraced. Dam of 7 foals to race, all winners, including-UNBEKNOWNST TO ME. 10 wins, 3 to 6, 3, 800, [Q] at Santa Anita Park twice, 2nd [Q] at Santa Anita Park, [Q] at Hollywood Park. NO PIRACY. 9 wins, 2 to 7 in Italy, Criterium Partenopeo, etc. Sire. Engagements: Oklahoma Classics. Accredited Oklahoma-bred.
A simple structure similar to previous Xilinx devices of a CLB Configurable Logic Block ; array, surrounded by programmable interconnect. The Virtex family, introduced in the late 90s, added large 4Kb ; BlockRAMs to the array and doubled the logic available in each CLB by including two slices per CLB, each slice being almost the same as an XC4000 CLB, providing for additional arithmetic support. The Virtex-II family was a further improvement on the Virtex architecture, increasing the size of the RAM blocks to 18Kb and including a fixed 18x18-bit multiplier with each RAM block. The logic density was doubled again, each CLB doubling in size to 4 slices, with additional support for logic functions being included. In addition to these, fast differential I O support was also built in. These families provided the basis for the Spartan range of low cost devices. The original Spartan family was based on the XC4000 family of high end devices. Similarly, the Spartan-II family is based on a cut down Virtex architecture and the Spartan-III's on the Virtex-II architecture. Table 10.9 shows the sizes and features available on each of these families. Within a family of devices only the number of each feature available, and the number and names of the I O pins, change. The features available remain the same, so a circuit targeted at one device will place and route on another device in the same family, provided that it is large enough. If I O pins are named within the circuit description, 343.
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2. Hormone activity is generally diminished. 3. The amount of body fat tends to increase with age. 4. CNS effects decrease in the elderly. Which nursing diagnosis would be the priority in the care of a patient with acquired immunodeficiency syndrome AIDS ; ? 1. Impaired skin integrity. 2. Anxiety death ; . 3. Pain acute ; . 4. Ineffective breathing pattern. Which of the following is most important to include in teaching a patient about preventing heat cramps when working outside in hot weather? 1. Prior history of heat sensitivity. 2. Need to maintain sodium level in body. 3. Importance of drinking fluids to stay cool. 4. Risk of potassium and magnesium loss. Which signs and symptoms indicate that the patient is experiencing hypovolemic shock? 1. Increased heart rate and decreased urine output. 2. Decreased urine output and pitting edema. 3. Fever and furrowed tongue. 4. Bounding pulse and crackles in the chest. Which sign is the best indicator of early infection in a patient who is immunosuppressed? 1. Fever. 2. Sore throat. 3. Elevated sedimentation rate. 4. Decreased urine output. The most important information to include when teaching a patient who is being discharged on continuous ambulatory peritoneal dialysis CAPD ; would be the need to: 1. Use sterile technique to prevent infection. 2. Increase protein in the diet. 3. Record blood pressure and weight daily. 4. Wear a medical identification bracelet. A patient complaining of severe indigestion while walking in the hall, grabs his chest and falls to the floor. The first assessment to make would be to determine: 1. Unresponsiveness. 2. Breathing pattern. 3. Blood pressure. 4. Presence of a pulse. A patient has hypotension, jugular venous distention, and muffled heart sounds on auscultation. The nurse would anticipate that the patient's treatment will be directed at: 1. Reversing cardiogenic shock. 2. Improving left ventricular function. 3. Reducing the degree of ventricular hypertrophy. 4. Managing pericardial effusion or tamponade. On admission of a patient with status epilepticus who has been having a seizure for over 2 hours, the most important assessment data would be: 1. Total time unconscious.
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System Reportedly in Place for Holding 2005 Elections in Haiti Organization of American States' Insulza lauds successful voter registration By Eric Green Washington File Staff Writer US State Deptartment 31 October 2005 Washington -- Haiti's "great success" in conducting a voter-registration campaign means that a system is now in place for the country to hold presidential and legislative elections during the first week of December, says Jos Miguel Insulza, secretary-general of the Organization of American States OAS ; . Insulza said the campaign has led to the registration of some 3.4 million eligible voters in Haiti as of October 26. "This means that we now have a system in place with which to carry out the elections, " Insulza said in an October 26 statement. However, he expressed concern over certain remaining organizational and logistical difficulties. Such difficulties caused a previous postponement of the elections. As an OAS spokesman explained, the elections in Haiti were originally scheduled for October and November, but the schedule was pushed back due to the complexity of organizing the vote. However, the plans to hand over power to a new democratically elected government on February 7, 2006, have not changed. The elections will be the first in Haiti since the country's former president, Jean-Bertrand Aristide, resigned his office and left the country in February 2004 in the wake of armed violence and political upheaval. In his statement, Insulza said, "The concern of the international community has been to ensure that the election takes place when it is supposed to take place -- that is, the first week of December." The United States is providing million to support Haiti's 2005 elections; in 2004, it provided .7 million to support Haiti's electoral process. See related article. ; Insulza's remarks follow those of U.S. State Department official Patrick Duddy, who has described Haiti's registration process as the "most comprehensive, transparent, and fraud-free ever conducted" in the history of the Caribbean nation. Duddy, deputy assistant secretary of state for Western Hemisphere affairs, said at an October 20-21 international donors' conference for Haiti that the over 3 million Haitians who have registered to vote will set the sage for broad participation in the elections. Duddy led the U.S. delegation to the donors' conference, held in Brussels, Belgium. See related article. ; The OAS's Insulza said his organization's support for Haiti's voter-registration campaign is designed to provide a "peaceful transfer of power [so] that we can begin a new stage in the history of Haiti.
A can of something has been cooking for 2 months under the sun near Abilene on a stretch of pavement not even the bikers care about. A pop-top all whose color has been bleached out. Maybe it was blue or could 'a' been green. It bulges as if about to pop but when I listen I hear only cicadas real far away and some other buzzing. What was put in there was made for people to drink--such an honor to become part of the top of the food chain. Now it is just some chemicals changing and waiting. This Winter a rain storm will wash it down into the arroyo, and another to the river, and then to the Gulf where it may wash up on a beach, be buried in sand and one day become the trophy in a very strange stranger's collection of impossibles and pitocin.
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2. VT, VF 3. Wide complex tachycardias of unknown origin. CONTRAINDICATIONS: 1. High degree HB's 2nd & 3rd ; 2. PVC's with bradycardia 3. Allergy to Medication or any "caines" 4. Idioventricular rhythms 5. Do not give with Beta-Blockers or Dopamine 1. Dosage not exceed 300 mg hr 2. Monitor for CNS toxicity 3. Reduc e dose by 50% in patients 70 or who have liver disease 4. Use bolus therapy in cardiac arrest only 1. Anxiety, drowsiness, dizziness, and confusion 2. Nausea, vomiting, & Convulsions 3. Widening of QRS 4. Hypotension, numbness, slurred speech, paresthesias ROUTE.
As predicted in the discussion, the patient's underlying SLE was manifested mainly as an active pericarditis and pleuritis. Figure 2 shows the microscopic appearance of the pericardium. This pericarditis is characterized by a dense, chronic inflammatory infiltrate, mostly lymphocytes and plasma cells, underlying a layer of organizing exudate. The exudate in this instance is purely fibrinous that is, nonpurulent ; . This rather bland chronic pericarditis is typical of lupus. A similar chronic inflammatory process attributable to the patient's SLE involved the pleura, but as predicted, we found a superimposed infectious process. A more-superficial exudate was found in the left pleural cavity. The leukocytes here were almost all neutrophils, in contrast to the lymphocytes and plasma cells associated with the more chronic lupus pleuritis; in fact, cocci were demonstrable in some parts of the exudate. Presumably this S pneumoniae infection reached the pleural space via a pulmonary portal of entry. The only other finding directly reflecting the patient's SLE was a trivial one, the "onion skin" periarteriolar fibrosis seen in the spleen. At the time of the patient's demise, the kidneys were not involved by lupus nephritis. They showed only the changes of nephrosclerosis correlating with the patient's history of long-standing hypertension. As expected, the immediate cause of death was found to be cardiac tamponade produced by hemopericardium. Figure 3 shows the surface appearance of the heart and aorta after draining the hemopericardium. Loculated, organizing hemorrhage is seen surrounding the base of the aorta and extending and posture!
Embolus was associated with paired infarcts of the caudoputamen and septal nuclei Figure 2, bottom ; . One hundred eighty-five cortical infarcts were observed in the territory of the anterior, middle, and posterior cerebral arteries, which in rats may all originate from the ICA.13 The hippocampus was involved unilaterally in three animals and bilaterally in one rat with massive bilateral infarcts. Two infarcts were found in the right brain stem and one in the right thalamus. The cerebellum was not examined. Ten large 2.5-13.5 mm ; cortical infarcts were seen, on the right in three, on the left in three, and bilaterally in two rats. While three of the large cortical infarcts appeared to be the coalescence of many smaller lesions, the others had a laminar pattern Figure 3 ; . The laminar pattern of neuronal necrosis was unilateral in three rats in two on the left and in one on the right ; and bilateral in two animals. None of the 19 control animals had infarcts, and no vascular occlusions were identified. Discussion We have demonstrated that emboli from the right CCA can go to both cerebral hemispheres in a rat if the left CCA is occluded. Occasionally, emboli from the carotid artery or embolic infarcts have been found in the opposite hemisphere, the brain stem, or the cerebellum in other models of embolic cerebral infarction in rats, 14 dogs, 15-17 and rabbits.18 In this model, where occlusion of one CCA results in a larger portion of the blood supply coming from the contralateral circulation via the circle of Willis, infarcts and emboli are relatively evenly distributed between the hemispheres, and we presume that emboli cross more frequently. Occlusion of the midline azygous artery in one third of our experimental animals supports our hypothesis that emboli in the vessels of the left hemisphere have crossed the midline from the right; in these cases crossing occurs via the anterior portion of the circle of Willis. Another explanation for the infarcts in the left hemisphere would be that they were caused by embolization of stagnated blood from the ligated ends of the artery, by mechanical manipulation of either CCA, or by absorption of the photosensitizing dye. Ligation of the left CCA, with and without Photofrin II injection, did not cause cerebral infarction in our control animals. Other investigators have reported that unilateral carotid artery ligation does not produce stroke in rats.19 Ninety percent of the brain lesions in our experimental rats were small and well-circumscribed, often associated with occluded vessels, consistent with the presumed mechanism of embolism.12 Evidence that cerebral infarcts are caused by emboli following photochemical damage to the carotid artery has been previously reviewed.12-14 Emboli were found in association with infarcts in both cerebral hemispheres, although the number of infarcts greatly exceeded the number of emboli detected. This study was not designed to locate all of.
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Thus, the decay time of the triplet state is very short 1 s ; and can be identified as the rise time of our luminescence signal at 1, 270 nm. With decreasing oxygen concentration, the deactivation of the triplet state by oxygen decreases and an increasing number of Photofrin molecules return to their own ground state. This leads to prolonged phosphorescence and an increase in the decay time of the triplet state. In the absence of oxygen, the luminescence signal measured is equivalent to the triplet emission of Photofrin, which is 200 s 15 ; . Thus, when we measure the time-resolved luminescence, the signal contains information about the oxygen concentration at the site of singlet oxygen generation, as either rise or decay time. At the lowest bacterial concentration, most of the bacteria are surrounded by water and there should be only a minor problem with oxygen supply if oxygen is consumed as a result of oxidative processes inside the bacteria. Thus, the decaying portion of luminescence should be attributed to the decay time of singlet oxygen 48 ; . However, with increasing bacterial concentration, the bacteria form large agglomerates, which hampers the oxygen support to all bacteria inside the agglomerates. When bacteria are irradiated in these agglomerates, the oxygen diffusion may be insufficient to compensate for the oxygen depletion during irradiation. Consequently, the oxygen concentration should decrease in the bacteria. As mentioned above, this oxygen decrease should lead to an increase in triplet decay time. At low oxygen concentrations, this triplet decay time can be identified as the decay time in our luminescence signal D 1 kD ; fact, with increasing bacterial concentration, kD is decreasing, which is equivalent to an increase in D Fig. 5 ; . This result can explain the quite extended decay time of the measured luminescence signal 40 s ; Fig. 5 ; . Because of this long-lasting phosphorescence signal, there is presumably no strong competitive process for triplet T1 deactivation in Photofrin, such as charge transfer type I reaction, oxygen radicals ; . To confirm this perception, conditions of low or high oxygen depletion in bacteria were created by exciting the photosensitizer for different durations. Low and high numbers of excitation pulses 8, 00080, 000 pulses ; were applied at the highest bacterial concentration 1.5 mg ml protein ; . In fact, the higher the number of pulses, the higher the oxygen depletion and, therefore, the longer the decay time of the luminescence signal Fig. 5 Inset ; . These results demonstrated that strong oxygen depletion takes place in the bacteria of agglomerates during irradiation, which should affect the efficacy of photodynamic inactivation of bacteria. When we extended the measurement of oxygen saturation up to 8 min, the value for low protein concentration was nearly constant and decreased slightly for high concentrations, which reflects the normal oxygen consumption by living bacteria Fig. 6, solid line ; . In the case where the laser was not switched off after luminescence detection, slight oxygen depletion was detectable for the low bacterial concentration and reached 0% for the high concentration Fig. 6, dashed line ; . Thus, remarkable oxygen consumption occurs as a result of the generation of singlet oxygen in bacteria, leading to a decrease in oxygen concentration in the entire bacterial suspension. Overall, the crucial point in efficient photodynamic killing of bacteria seems to be the oxygen concentration inside the bacteria at a given bacterial concentration during the irradiation process. If bacteria grow in agglomerates or biofilms with an insufficient supply of oxygen during photodynamic inactivation, efficacy in bacterial killing should be considerably attenuated. Indeed, when the viability of S. aureus was measured after an irradiation period of 6 min, the cfus decreased for the low bacterial concentration by a factor 20 but for the high bacterial concentration only by a factor 8. This finding additionally confirms the better oxygen supply for the bacteria surrounded by water Fig. 1 A ; than for bacteria in agglomerates Fig. 1D and praziquantel.
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The preferred photosensitizing agent for use in the course of the present invention is photofrin product of sanofi pharmaceuticals, inc, new york, new york and prevnar.
Ou lost a dog, I don't know how, or where, but I do know when: last November. Or, you gave him up, or maybe even you died--I don't know which. All I do know is that he was somebody's--a well-behaved dog with perfect manners. I also know that he ended up in the county pound down south, where when his time ran out some kind-hearted people called a rescue group rather than see him put down. By this time, though, his weight was half what it should have been and he was allergic to his food. His long coat was matted and rough, and his body wracked with hookworm and bacterial infections. His eyes were dull, his nose was hot, and at about 7 years old, I think his heart was broken. He ended up in the rescue group's shelter where I found him about a week later. I had dreamed of a dog the previous night, and I know this sounds strange ; my son and I went looking for him. The rescue group had done a good job on his coat, and he was fed and reasonably warm . but still, when I met him, his eyes and spirit were dull like someone who doesn't dare to hope. He rose on his back legs and put his paws on my waist, and looked and looked. And I was smitten. In my heart he became my dog that day, although I didn't get to take him home until I'd done the application, had the home visit and paid the adoption fee. I'd do all those things again for this dog, by the way--and much more if more were needed. I picked him up on a day when a blizzard was due, and took him home. He got a new name, and a new life. Slowly he has come back to health--he got the vet treatment he needed, and special hypoallergenic food, and daily coat brushings. All the infections are just a bad memory now. His weight has doubled, his coat is glossy and silky and his eyes sparkle. I think we've filled the empty places in his broken heart. He runs like the wind and dances at dinnertime. So I just want you to know: He is safe, warm and loved. He will never be in need again. So I just wanted to say to you and to the rescuers, "Thank you for my dog". I know many dog stories don't end happy, but I just wanted you to know, for this one it did. He's home, he's safe and he's loved . forever. author unknown, submitted by Anne Byrd.
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